Exoenzyme Profiling of Soil Bacteria from Thattekad Bird Sanctuary for Bioprospection

Thattekad bird sanctuary, located in the Western Ghats of Kerala, India, which hosts an unexplored microbial community, is selected for present investigation. Microbes play a major role mineral recycling and nutrient absorption by flora fauna habitat. Various bacterial extracellular enzymes facilitate all these activities. The increasing demand favor green technology encouraged us to focus on exoenzyme profiling isolates from forest soil samples. study aimed at screening identification producing strains isolated evergreen forests moist deciduous sanctuary. In this study, only multienzyme bacteria were detailed analysis because such are highly relevant multi-enzyme dependent processes as biowaste degradation. We screened nine hydrolytic exoenzymes namely, amylase, cellulase, ligninase, pectinase, xylanase, caseinase, gelatinase, esterase lipase, identified 79 multienzyme-producing strains, mostly belonging phylum Firmicutes Proteobacteria. produced greater number compared Also, more forest. Bacillus amyloliquefaciens strain TBS040 was found produce screened. Enzymatic hydrolysis using cell free crude enzyme extract velezensis TBS064 resulted enhanced bioethanol production. These findings highlight importance habitats novel can contribute future technology.